primary antibody rabbit anti-perk  (PhosphoSolutions)

Journal: International Journal of Molecular Sciences

Article Title: Pharmacological Inhibition and Genetic Deletion of Cystathionine Gamma-Lyase in Mice Protects against Organ Injury in Sepsis: A Key Role of Adhesion Molecules on Endothelial Cells

doi: 10.3390/ijms241713650

Figure Lengend Snippet: Primary and secondary antibodies used in this study for Western blotting and immunofluorescence microscopy.

Article Snippet: ERK1/2 , Primary/Rabbit monoclonal , Cell Signaling, Danvers, MA, USA/137F5 , 1:2000.

Techniques: Western Blot, Immunofluorescence, Microscopy

Journal: The FASEB Journal

Article Title: Exogenous hydrogen sulfide alleviates hepatic endoplasmic reticulum stress via SIRT1/FoxO1/PCSK9 pathway in NAFLD

doi: 10.1096/fj.202201705rr

Figure Lengend Snippet: FIGURE 4 GYY4137 relieved lipid accumulation, elevated H2S level and reduced ER stress in HepG2 cells induced by LM. The HepG2 cells exposed to 10% LM were treated with or without GYY4137 (200 μM) for 24 h, and then, the cells were collected for further detection. (A) Lipid droplets were visualized with Oil Red O staining (2.5 μm). (B) Cellular TG and TC levels. (C) Relative mRNA levels of PPAR-γ, ACC, and SREBP-1c by qRT-PCR. (D) The level of cellular H2S. (E) Western blot analysis of CSE and α-Tubulin used as a loading control. (F) The cells' morphology of ER marked with red arrows was observed by TEM (5 and 2 μm). (G) Western blot analysis of ER stress markers (PERK, eIF2α, GRP78, p-PERK, and p-eIF2α) and α-Tubulin used as a loading control. Data were presented as mean ± SEM (each experiment repeated at least three times independently). *p < .05, **p < .01 versus control or GYY (200); #p < .05, ##p < .01 versus LM. ER, endoplasmic reticulum; LM, lipid mixture; qRT-PCR, quantitative real-time PCR; TC, total cholesterol; TEM, transmission electron microscope; TG, triglyceride. GYY (200), GYY4137 (200 μM).

Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Rabbit anti- α- Tubulin primary antibody (Cat#11224- 1- AP, RRID:AB_2210206), rabbit anti- SIRT1 primary antibody (Cat#13161- 1- AP, RRID:AB_10646436), rabbit anti- CSE primary antibody (Cat#12217- 1- AP, RRID:AB_2087497), rabbit anti- PERK primary antibody (Cat#20582- 1- AP, RRID:AB_10695760), rabbit anti- GRP78 primary antibody (Cat#11587- 1- AP, RRID:AB_2119855), rabbit anti- eIF2α primary antibody (Cat#11170- 1- AP, RRID:AB_2096489), rabbit anti- p- eIF2α primary antibody (Cat#28740- 1- AP, RRID:AB_2881204), rabbit anti- PCSK9 primary antibody (Cat#27882- 1- AP, RRID:AB_2918134) and mouse anti- acetylation primary antibody (Cat#66289- 1- Ig, RRID:AB_2881672) were purchased from Proteintech (Wuhan, China).

Techniques: Staining, Quantitative RT-PCR, Western Blot, Control, Real-time Polymerase Chain Reaction, Transmission Assay, Microscopy